96 Well Cell & Bacterial Culture Plate

Multi-well plates are disposable, sterile plastic plates containing an array of individual wells. They are foundational tools for high-throughput biological research, enabling the parallel culture of cells or bacteria, execution of assays, and screening of compounds under multiple conditions in a single, standardized format. This drastically increases experimental scale and reproducibility.

The choice balances sample volume, required cell mass, and experimental throughput: 6-Well Plates: Offer the largest well surface area and volume. Ideal for expanding cell stocks, transfections, RNA/protein harvest for downstream analysis (e.g., Western blot), and colony formation assays. 12 & 24-Well Plates: Provide a versatile mid-range scale. Perfect for immunocytochemistry, mid-throughput cytotoxicity/drug testing, bacterial protein expression screening, and assays requiring more material

The key difference is often the surface treatment: Cell Culture Plates (for Mammalian Cells): Most are Tissue Culture (TC)-Treated. This creates a hydrophilic, negatively charged surface that promotes the attachment and spreading of adherent cells. For suspension cells, non-treated plates are used. Bacterial Culture Plates: Use non-treated, standard polystyrene. Bacteria do not require the charged surface for attachment in liquid culture. Special deep-well plates (with 1-2mL well volume) are o

For Mammalian Cells: Plates must be placed in a humidified, 5% CO2 incubator at 37 C de. To minimize evaporation from edge wells ("edge effect"), consider placing the plate in a secondary container with sterile water or using a plate sealer. For Bacteria: Incubation is typically in a standard, non-humidified bacterial incubator at 37C (or other required temperature) with shaking for liquid cultures. Sealing the plate with a breathable membrane or lid is crucial to prevent contamination and dehy